Hi Anil,
Have you added like below?
#R3 COnnection
KP "p:CN=sapserv2, OU=SAProuter, O=SAP, C=DE" <IP> 32<inst no>
# WTS Connection.
KP "p:CN=sapserv2, OU=SAProuter, O=SAP, C=DE" <IP> 3389
#Http
KP "p:CN=sapserv2, OU=SAProuter, O=SAP, C=DE" <IP> 5<inst no>00
and check if any internal firewall blocks if still issue persists .
Regards,
Nibu Antony